Enzyme Activity & Specific Activity

Calculate enzyme activity in units and specific activity per milligram from a rate assay, free online.

Change in product concentration (µM)

Time (min)

Reaction volume (mL)

Enzyme protein in the assay (mg)

About this calculator

One unit (U) of enzyme converts 1 µmol of substrate per minute. Activity = Δconcentration × reaction volume ÷ time. Specific activity = units ÷ mg of protein, a measure of enzyme purity.